| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003711 |
| Chromosome: | chromosome 12 |
| Location: | 6015610 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g534450 | CGL86 | forkhead-associated domain-containing protein; (1 of 1) PTHR23308//PTHR23308:SF29 - NUCLEAR INHIBITOR OF PROTEIN PHOSPHATASE-1 // SUBFAMILY NOT NAMED | 3'UTR |
| Cre12.g534500 | (1 of 1) PTHR20955//PTHR20955:SF1 - UNCHARACTERIZED // PROTEIN JAGUNAL HOMOLOG 1 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGGGATGAGGGTGCCGGACCGTGGTCAGGGTTGCAGCAGCAGCTGCTGC |
| Internal bar code: | CTCGAATTAGACCAGGAATCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4372 |
| LEAP-Seq percent confirming: | 78.2609 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 25 |
| LEAP-Seq n unique pos: | 115 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTGTGCCACTGTGCTGAAG |
| Suggested primer 2: | TACGGCCTTACACACCACAC |