| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | CLIP2.003733 |
| Chromosome: | chromosome 6 |
| Location: | 4321087 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g278264 | (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCGCGGCCCCGTACACCACATTACAGCAGCATTACGTGATGAATCATTT |
| Internal bar code: | AATGGGTGCCTTACAGATGATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1588 |
| LEAP-Seq percent confirming: | 93.75 |
| LEAP-Seq n confirming: | 60 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 64 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATACTTGTCCACCGTGCGT |
| Suggested primer 2: | TTCGCCGACTGTCCTACAAC |