Insertion junction: CLIP2.003739_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003739
Chromosome:chromosome 12
Location:2039152
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g510400 RBD4,CPLD30 Conserved in the Plant Lineage and Diatoms; (1 of 4) IPR024934 - Rubredoxin-like domain intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCGAGCGCGCGCATCTGTAGCTGTGCCAGACGCTATGCTGACGTCGCC
Internal bar code:ACTATTTCGATTAACTCCTAGC

Confirmation - LEAP-Seq

LEAP-Seq distance:6056
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:82
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:82

Suggested primers for confirmation by PCR

Suggested primer 1:TAATCGCCAGAACAGCAGCA
Suggested primer 2:AAGCTCGAACCTGGCTCATC