| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003771 |
| Chromosome: | chromosome 10 |
| Location: | 2251433 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g433900 | (1 of 2) K10592 - E3 ubiquitin-protein ligase HUWE1 (HUWE1, MULE, ARF-BP1) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAACGGCCGCTACTTCCACGTCACCAACCTGTCCAACTGGTCCATGG |
| Internal bar code: | TCAATTATGGGATAATTCGAGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3086 |
| LEAP-Seq percent confirming: | 97.2973 |
| LEAP-Seq n confirming: | 36 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 37 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GATGCATATGCCTGGACGGA |
| Suggested primer 2: | TGGGTGTACGGTCTTTGCTC |