Insertion junction: CLIP2.003784_1

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.003784
Chromosome:chromosome 6
Location:6882413
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g296550 FMO3 Flavin-containing monooxygenase; (1 of 3) K00485 - dimethylaniline monooxygenase (N-oxide forming) (FMO) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AGGTGCCGTGTGTCTTTGCGCGCAGTGCCTCATCGCGCGCCAGTTCCCCG
Internal bar code:CTAAAAATTGAACAGCCTCCCA

Confirmation - LEAP-Seq

LEAP-Seq distance:2099
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:3
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:3

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGACACCTGTTGGTTTGC
Suggested primer 2:CTCGCTCACACGACAACTCT