| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003786 |
| Chromosome: | chromosome 5 |
| Location: | 809098 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g245900 | BCA2 | (1 of 1) PTHR11825//PTHR11825:SF46 - SUBGROUP IIII AMINOTRANSFERASE // BRANCHED-CHAIN-AMINO-ACID AMINOTRANSFERASE 3, CHLOROPLASTIC; Branched-chain amino acid aminotransferase | outside_mRNA |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CATGTGTGACCTCCATCCAGCAGCTGCCAGCGCACCCTGCCGCCGCAGAT |
| Internal bar code: | AGGGGGAGTGATAAACACGTTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1040 |
| LEAP-Seq percent confirming: | 95.4545 |
| LEAP-Seq n confirming: | 21 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTCTGGAGATGTACTCGGC |
| Suggested primer 2: | TCCGATATGCTCCCGATTGC |