Insertion junction: CLIP2.003791_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.003791
Chromosome:chromosome 16
Location:4534573
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g671500 (1 of 781) IPR000104 - Antifreeze protein, type I intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCTCCTTCAGCCGCCCCCACACACACCTCCTTCAGCCGCCCGCGGTCT
Internal bar code:GCCGAATTGATGATAATTTTCG

Confirmation - LEAP-Seq

LEAP-Seq distance:3600
LEAP-Seq percent confirming:97.3333
LEAP-Seq n confirming:73
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:75

Suggested primers for confirmation by PCR

Suggested primer 1:CTGGCTCGCTGGTTGAGTTA
Suggested primer 2:GTCTGAGGCAACAGCAGAGT