| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003842 |
| Chromosome: | chromosome 3 |
| Location: | 6658226 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g195200 | HLD1,CGL76 | Conserved in the Green Lineage; (1 of 6) IPR000073//IPR000639//IPR029058 - Alpha/beta hydrolase fold-1 // Epoxide hydrolase-like // Alpha/Beta hydrolase fold | 5'UTR |
| Cre03.g195250 | (1 of 2) PF13679 - Methyltransferase domain (Methyltransf_32) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACACCCCACCAGTCAGTGGGGACACCCCCGGTGCCAACGCATTCTGAAGT |
| Internal bar code: | GTTATGGGAGATGAGTCGGCAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3462 |
| LEAP-Seq percent confirming: | 94.8052 |
| LEAP-Seq n confirming: | 73 |
| LEAP-Seq n nonconfirming: | 4 |
| LEAP-Seq n unique pos: | 77 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTGTGTCACTGGCAGCTA |
| Suggested primer 2: | TGGGGTCGATTTCCTCAAGC |