| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003881 |
| Chromosome: | chromosome 3 |
| Location: | 143622 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g143947 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAACTACGCTATGTGGGGTGTCTTGATCGCGATGTGCCTGCTGAGCAC |
| Internal bar code: | CGAGCGGTATCTACTTGAGGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3913 |
| LEAP-Seq percent confirming: | 95.5556 |
| LEAP-Seq n confirming: | 43 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCACCTTCAATCTGGACCGC |
| Suggested primer 2: | TCGTGAATGCTTTCTCCCCC |