Insertion junction: CLIP2.003884_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.003884
Chromosome:chromosome 10
Location:4157229
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre10.g448950 EEP3 Exonuclease-Endonuclease-Phosphatase superfamily protein; (1 of 1) K18764 - nocturnin [EC:3.1.13.4] (CCRN4L) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCGACACGTCCCCGGTTCCCTAGGTACACCCCGACCACCTCAAATGGGA
Internal bar code:GCGATCCCTACCTGCAGTAACT

Confirmation - LEAP-Seq

LEAP-Seq distance:3970
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ATGTCAAGCCTCGATCACGG
Suggested primer 2:CAATCCCGCGATCGCAAATT