Insertion junction: CLIP2.003907_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003907
Chromosome:chromosome 3
Location:5551280
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre03.g185250 SSS2,SSII (1 of 1) IPR001296//IPR011835//IPR013534//IPR015421 - Glycosyl transferase, family 1 // Bacterial/plant glycogen synthase // Starch synthase, catalytic domain // Pyridoxal phosphate-dependent transferase, major region, subdomain 1; Soluble starch synthase II CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGAGGTGATCAAGGTGAACAACTGGAAGCTGCGCGGCATTGTGAACGG
Internal bar code:GATCCAATTATGATGACGGTAG

Confirmation - LEAP-Seq

LEAP-Seq distance:4632
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:149
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:149

Suggested primers for confirmation by PCR

Suggested primer 1:ACCACGATAGCCCTGAGACT
Suggested primer 2:TGGGCTACTTCCACGCATTT