| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.003910 |
| Chromosome: | chromosome 7 |
| Location: | 5152729 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348550 | TGL13 | (1 of 3) PTHR21493:SF153 - PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGGTGTTCTTGGGGTACCAGCTGCTGCCGTCGGGCAGGCGAGCCGCCTT |
| Internal bar code: | TTAGCGGGGGTTGTCCCGCTTA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3727 |
| LEAP-Seq percent confirming: | 86.0215 |
| LEAP-Seq n confirming: | 80 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 93 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTTACGTCGTGCGCATG |
| Suggested primer 2: | ACACACACACACACACGTCT |