Insertion junction: CLIP2.003920_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003920
Chromosome:chromosome 7
Location:3532898
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAGATCCGTTTCCCTTACCCGCTCACAGGTTCTCCCTGCCAGGGGTATC
Internal bar code:GACGCGATAACTAGTTGCCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:5485
LEAP-Seq percent confirming:96.1538
LEAP-Seq n confirming:100
LEAP-Seq n nonconfirming:4
LEAP-Seq n unique pos:104

Suggested primers for confirmation by PCR

Suggested primer 1:CGCATCCCACGTGTTTGTTT
Suggested primer 2:CAGGTGTGAAGTACGGCAGT