Insertion junction: CLIP2.003920_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.003920
Chromosome:chromosome 7
Location:3532900
Confidence (%):80
Locus systematic id Locus common name Defline Feature
intergenic

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCAAGCAGAACGCTCGCTGACTTGTTGCCAGTAGCTGCCGTAGCTGACTG
Internal bar code:GACGCGATAACTAGTTGCCTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:2970
LEAP-Seq percent confirming:98.9247
LEAP-Seq n confirming:92
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:93

Suggested primers for confirmation by PCR

Suggested primer 1:CAGGTGTGAAGTACGGCAGT
Suggested primer 2:CGCATCCCACGTGTTTGTTT