Insertion junction: CLIP2.003941_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.003941
Chromosome:chromosome 2
Location:1309834
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre02.g082550 ZEP,ZEP1 Zeaxanthin epoxidase 1; (1 of 1) K09838 - zeaxanthin epoxidase (ZEP, ABA1) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGTTTCCGGCCTTGTCGGACACGGCGCGGCTGAGGTCGATGGCCAGCTC
Internal bar code:GTAGTGGATTCCACATGACTAC

Confirmation - LEAP-Seq

LEAP-Seq distance:4425
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:105
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:105

Suggested primers for confirmation by PCR

Suggested primer 1:CAACGTGGTGGACCTGATCA
Suggested primer 2:TGTTGTGGTTTTGGGGACGA