Insertion junction: CLIP2.003957_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:+
Strain:CLIP2.003957
Chromosome:chromosome 9
Location:5625259
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g410000 (1 of 1) PTHR13604//PTHR13604:SF0 - DC12-RELATED // EMBRYONIC STEM CELL-SPECIFIC 5-HYDROXYMETHYLCYTOSINE-BINDING PROTEIN intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATGTGCCTCGGGCGTGTGTGTTGGAGTTTGTAGTTGTAGGATGGGTGGAG
Internal bar code:TAAAGGGAACGAGCAGTGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:1581
LEAP-Seq percent confirming:85.7143
LEAP-Seq n confirming:18
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:21

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCACACAAACTTGGTGCG
Suggested primer 2:CACCATCATCACCACCGACA