Insertion junction: CLIP2.003960_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.003960
Chromosome:chromosome 12
Location:6311819
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g536800 CGL52 (1 of 8) PF10184 - Uncharacterized conserved protein (DUF2358) (DUF2358); Conserved in the Green Lineage intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACCAACCCCACCAACACCCACGCTGCCCCCTCCCTCCCGCCCACCTTAT
Internal bar code:CACTATTTTTTTACCTATCGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:78
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:5
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:ACTGAGGCTGCAATTCCCTC
Suggested primer 2:CTTTGCGTGCTTACCCAACC