Insertion junction: CLIP2.003969_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003969
Chromosome:chromosome 12
Location:4542184
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g521300 POB14 Proteome of basal body 14 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TAAAGCATCGACACTGACGTGGACCCGAAACACTTTCCAGGCACCTTCGG
Internal bar code:TCTCTGGTAATTTGGAGACATT

Confirmation - LEAP-Seq

LEAP-Seq distance:3521
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:59
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:59

Suggested primers for confirmation by PCR

Suggested primer 1:GCAGGTTATGCGACAGCTTG
Suggested primer 2:GCCATGCGCAACTAAGCTAC