Insertion junction: CLIP2.003973_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003973
Chromosome:chromosome 9
Location:5334082
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g407600 TEN1 Conserved TenA familiy member; (1 of 1) PTHR20858//PTHR20858:SF18 - PHOSPHOMETHYLPYRIMIDINE KINASE // PROTEIN PET18 3'UTR
Cre09.g407650 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTCTGGTCAACCCGTCGATCCACGGCTGGTGCGGCTGCGGGAGCCACACA
Internal bar code:TCTGACGACGGCCAGTATTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:1376
LEAP-Seq percent confirming:96.5517
LEAP-Seq n confirming:28
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:AAATGTGGCGTGACGTGTTG
Suggested primer 2:GTTGTCAAGAACACGGGCAC