Insertion junction: CLIP2.003973_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.003973
Chromosome:chromosome 9
Location:5334125
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g407600 TEN1 Conserved TenA familiy member; (1 of 1) PTHR20858//PTHR20858:SF18 - PHOSPHOMETHYLPYRIMIDINE KINASE // PROTEIN PET18 3'UTR
Cre09.g407650 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCACTCAAATCCTAACAAGGACGTGGCGTGACGTGCGCGGTGCGCCAAGT
Internal bar code:TCTGACGACGGCCAGTATTGCC

Confirmation - LEAP-Seq

LEAP-Seq distance:734
LEAP-Seq percent confirming:92.8571
LEAP-Seq n confirming:26
LEAP-Seq n nonconfirming:2
LEAP-Seq n unique pos:28

Suggested primers for confirmation by PCR

Suggested primer 1:GTTGTCAAGAACACGGGCAC
Suggested primer 2:AAATGTGGCGTGACGTGTTG