Insertion junction: CLIP2.003984_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.003984
Chromosome:chromosome 1
Location:123996
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre01.g000900 CPLD20 Conserved in the Plant Lineage and Diatoms; (1 of 2) PF07082 - Protein of unknown function (DUF1350) (DUF1350) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CACGACACACGACACAGTACAAACGCGTGTTTCAGTGCACGAACACGCCA
Internal bar code:GTGTTTTTCATATGGGCAGGTT

Confirmation - LEAP-Seq

LEAP-Seq distance:48
LEAP-Seq percent confirming:50.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:AGTGTCTTGCCCCTTCCTTG
Suggested primer 2:TGTGGCCCCGGTAAACAATT