Insertion junction: CLIP2.003996_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.003996
Chromosome:chromosome 14
Location:2059045
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre14.g621351 (1 of 2) 2.7.6.5 - GTP diphosphokinase / Stringent factor CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTCCATGGCGCCCTCGGAACGAGAGTTCCTCCGCTACGCCATCTGCTGCA
Internal bar code:CACTATTGGACCAGTGTTACTC

Confirmation - LEAP-Seq

LEAP-Seq distance:2094
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:62
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:62

Suggested primers for confirmation by PCR

Suggested primer 1:CCGATGAGAGGTCTGATGCC
Suggested primer 2:GTGGCCATGAGGTTGTAGCT