| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004004 |
| Chromosome: | chromosome 16 |
| Location: | 257192 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g694400 | TGD2,CGL57 | Trigalactosyldiacylglycerol 2; (1 of 2) PF02470 - MlaD protein (MlaD) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCACTGGCTGCACCCCCAGGTCGCCCAGGTCGTTGCAGGCATGTAGTGGC |
| Internal bar code: | TAAACGTTCGCAGTGGTCCCTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 942 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 23 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 23 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACGGCTGAACCACTTGTTG |
| Suggested primer 2: | TGTTGAAGCCCGCCAAATTG |