| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004007 |
| Chromosome: | chromosome 12 |
| Location: | 2814826 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g503550 | MEC1,MCS,MECPS1 | 2-C-methyl-D-erythritol 2%252C4-cyclodiphosphate synthase; (1 of 1) 4.6.1.12 - 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase / MECDP-synthase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATATCAAAAATGGCCCTTCAGTCCCTTCGTGGTCGTTCTGCCTTCTCCAG |
| Internal bar code: | ATATAGATAAGCTCATCTGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2649 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 62 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 62 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGATGTCGATACCGCCAAT |
| Suggested primer 2: | TGAGGTGTGTGATGTCGGTG |