| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004038 |
| Chromosome: | chromosome 7 |
| Location: | 6098109 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g355466 | AGE2 | DNA repair glycosylase; (1 of 1) PF15628 - RRM in Demeter (RRM_DME) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCGGACCAAGAATTGTGTGTGTATGCGTGTGTGTCTGGGGGGGTTGTAAA |
| Internal bar code: | GTTAGTCCGACACAGGTCTACT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1743 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAACTCCTGCTAATGCGCCT |
| Suggested primer 2: | CTCTAGAGCTACTGCCGCAC |