| Insertion cassette: | CIB2 |
| Side of cassette: | 5' truncated? |
| Strand: | + |
| Strain: | CLIP2.004106 |
| Chromosome: | chromosome 1 |
| Location: | 3468222 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g022550 | (1 of 15) 3.6.5.5 - Dynamin GTPase | 3'UTR | |
| Cre01.g022600 | CGL13 | Conserved in the Green Lineage; (1 of 2) 2.8.2.20 - Protein-tyrosine sulfotransferase / Tyrosylprotein sulfotransferase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAGCTAGGCACTTCCTTGGATGGTGCAATTTCGGTTGTGACTTGACTGCA |
| Internal bar code: | GATCGTAGTGTTTGCGTCCAGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1141 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 24 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCATTTCCCTTCTCCGACC |
| Suggested primer 2: | GACAATGGGAGTGGAAGGGG |