Insertion junction: CLIP2.004116_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004116
Chromosome:chromosome 9
Location:1472988
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre09.g399914 SSA8 cilia-sensing, structure and/or assembly; (1 of 1) K10766 - alkylated DNA repair protein alkB homolog 4 (ALKBH4) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCGCCTGCGACACCGGCCTGCGCCTGCTGGTGCTGCTGGTGCTCCTGCTG
Internal bar code:AGAAGGGACCTGCCTATCCAAC

Confirmation - LEAP-Seq

LEAP-Seq distance:1985
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:TCATGTGGAATTGGTGCCGA
Suggested primer 2:TGTCGTGTGCGTCATTCAGA