| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004124 |
| Chromosome: | chromosome 3 |
| Location: | 3003722 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g163350 | PAN5,PANE | (1 of 1) 1.1.1.169 - 2-dehydropantoate 2-reductase / KPA reductase; Ketopantoate reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGCGGGCTGCTGGTGGGTAGCATGATCTGGATGACGGCGATCGTGATCG |
| Internal bar code: | GGGATGGTTCAAAGGATGTGAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3609 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 35 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 35 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCCCCATTTTCCCTGACCG |
| Suggested primer 2: | CCCAGACCAGCTGCCATAAA |