Insertion junction: CLIP2.004126_2

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:+
Strain:CLIP2.004126
Chromosome:chromosome 6
Location:2575319
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269752 TRXm,TRXM1,TRX15 (1 of 1) IPR000104//IPR005746//IPR012336//IPR013766 - Antifreeze protein, type I // Thioredoxin // Thioredoxin-like fold // Thioredoxin domain; Thioredoxin m, chloroplastic 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ATTTCTTCTCCACGCGGCATCAACGCCAACTACAGACGGCCTTTCATCTC
Internal bar code:TTCGATGGGTTATGTATGGTGG

Confirmation - LEAP-Seq

LEAP-Seq distance:3218
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:20
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:20

Suggested primers for confirmation by PCR

Suggested primer 1:CTGAGCATTGCGGTTGATCG
Suggested primer 2:GCTAACGTGGTCCCCAATCA