| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004135 |
| Chromosome: | chromosome 2 |
| Location: | 7999827 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g145000 | RBF1 | ribosome-binding factor A; (1 of 1) K02834 - ribosome-binding factor A (rbfA) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGGGTACGCGGCAGTATGGCGCTGCAGCGCGGCGCAGCCACTGGCACCT |
| Internal bar code: | GTGAGGGTTCACCTTTGCGGGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 888 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGCAGTAGGCCAGTAGCTCT |
| Suggested primer 2: | TGCAGGTGGTCAAGGTGTTT |