Insertion junction: CLIP2.004139_1

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:-
Strain:CLIP2.004139
Chromosome:chromosome 5
Location:2794919
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre05.g241000 (1 of 2) PF10084 - Uncharacterized protein conserved in bacteria (DUF2322) (DUF2322) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GCCCCCTCCTCCCTTCTCCCCTTCTCCCTCGTCCCATCCCTCCCTGCCCT
Internal bar code:CGCTGCGTACTTTCGGTCATTT

Confirmation - LEAP-Seq

LEAP-Seq distance:3390
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:39
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:39

Suggested primers for confirmation by PCR

Suggested primer 1:TCTCGTGCTCCCTCAAACAC
Suggested primer 2:GACTGGAGAGAATACGGCGG