| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.004158 |
| Chromosome: | chromosome 5 |
| Location: | 378169 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre05.g243550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTCGTGCGGATGCCAGGATAGTCCCAGCACACGTGTAGTGCCGACCAGT |
| Internal bar code: | AGGATCGCAATACGAAATAAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3749 |
| LEAP-Seq percent confirming: | 89.2308 |
| LEAP-Seq n confirming: | 58 |
| LEAP-Seq n nonconfirming: | 7 |
| LEAP-Seq n unique pos: | 65 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCCAATGCTACCCACGAA |
| Suggested primer 2: | CCATTCAAGGTGCGCTATGC |