| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | CLIP2.004160 |
| Chromosome: | chromosome 7 |
| Location: | 901805 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g318850 | HSP22B | heat shock protein 22B; (1 of 2) K13993 - HSP20 family protein (HSP20) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CAATCCTGAATACCCGAACGACAATTTACAAGGACCGGCAAGACTACACA |
| Internal bar code: | CTCTCTTAGCCCTCATACTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2336 |
| LEAP-Seq percent confirming: | 83.3333 |
| LEAP-Seq n confirming: | 5 |
| LEAP-Seq n nonconfirming: | 1 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTAGCTTTCAATGCGCCGAG |
| Suggested primer 2: | GCTTCGTTGCATGGGCTATG |