Insertion junction: CLIP2.004174_1

Overview

Insertion cassette:CIB2
Side of cassette:5'
Strand:-
Strain:CLIP2.004174
Chromosome:chromosome 6
Location:5242078
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g284100 RHP1,RH1 Rhesus protein, CO2-responsive; (1 of 2) K06580 - ammonium transporter Rh (SLC42A, RHAG, RHBG, RHCG) CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGCCTTCCCTCTCGCGAGACGCAGCTGCGGGCTGGCTTTGTGTGAGTCGT
Internal bar code:TTAGTCGAGGTCGGTGTCTAAG

Confirmation - LEAP-Seq

LEAP-Seq distance:6297
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:117
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:117

Suggested primers for confirmation by PCR

Suggested primer 1:AAGGACCTGGACCTATCCCC
Suggested primer 2:GGTGCGTCGAAATCGTCATG