| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | - |
| Strain: | CLIP2.004216 |
| Chromosome: | chromosome 16 |
| Location: | 5531042 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g682100 | TDR1 | (1 of 1) 1.1.1.206//1.1.1.236 - Tropinone reductase I / Tropinone reductase // Tropinone reductase II / Tropinone reductase; Tropine dehydrogenase/reductase | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TCCTCCGTCGCGGGCGGCCCCACCGCCATGAGGTGGGGGGGTTACATTCA |
| Internal bar code: | GGGCCGTTATAAACGTTACGCA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1217 |
| LEAP-Seq percent confirming: | 85.0 |
| LEAP-Seq n confirming: | 17 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 20 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTCATACGTGGTCGCGAG |
| Suggested primer 2: | GTGGAGTACTCGGAGGAGGT |