| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.004219 |
| Chromosome: | chromosome 10 |
| Location: | 6658722 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g466250 | PRP20 | (1 of 1) K14554 - U3 small nucleolar RNA-associated protein 21 (UTP21, WDR36); Putative Utp21 subunit of U3 snoRNP | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCAGCGTTTGCCCCCACAGGAAATGCGGCTTGTGGGAAACAGGTATCG |
| Internal bar code: | TCAGTAAGACAGTACAAAACGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1174 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 4 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCGGCAAGAACTTCCAGGTC |
| Suggested primer 2: | GCACACAAACACATAGCGCA |