Insertion junction: CLIP2.004221_2

Overview

Insertion cassette:CIB2
Side of cassette:3' truncated?
Strand:+
Strain:CLIP2.004221
Chromosome:chromosome 12
Location:8558385
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre12.g548950 LHCBM7 (1 of 3) K08913 - light-harvesting complex II chlorophyll a/b binding protein 2 (LHCB2); Light-harvesting Chlorophyll a/b binding protein of LHCII intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CTTTACCTTTGTCATGTCGTTTGCTTGCAGGCCCCTTCTCGGAGGGCGAC
Internal bar code:TAGTTAAAGCATATGTAGGCCT

Confirmation - LEAP-Seq

LEAP-Seq distance:1546
LEAP-Seq percent confirming:57.1429
LEAP-Seq n confirming:4
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:7

Suggested primers for confirmation by PCR

Suggested primer 1:AAATTTAGGCCGAGGGGGTG
Suggested primer 2:CTCCGAGACATCCATCCACG