Insertion junction: CLIP2.004244_2

Overview

Insertion cassette:CIB2
Side of cassette:5' truncated?
Strand:+
Strain:CLIP2.004244
Chromosome:chromosome 6
Location:2517266
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre06.g269300 CGL120 (1 of 1) PF07103 - Protein of unknown function (DUF1365) (DUF1365); Conserved in the Green Lineage 120 intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCTTTTCCAAACATCCTTGCAACGCCACCCCGCCCCATCCCTCCGCCGC
Internal bar code:TATTTTTAAGTTGTTAGCGGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:1325
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:12
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:12

Suggested primers for confirmation by PCR

Suggested primer 1:CCCCTTGATGAGCAGCTTCA
Suggested primer 2:TCAACTGAGCACTCCGGTTC