| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004253 |
| Chromosome: | chromosome 2 |
| Location: | 1103310 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g080650 | HSP90B | (1 of 1) PTHR11528//PTHR11528:SF45 - HEAT SHOCK PROTEIN 90 // ENDOPLASMIN HOMOLOG; Heat shock protein 90B | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCCAACACACGCGGCAGACTGGCGCGGACACTCCAGCAGCAGCAGGCAAC |
| Internal bar code: | CGCGTGATGTGTGATCGAATGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1254 |
| LEAP-Seq percent confirming: | 67.8571 |
| LEAP-Seq n confirming: | 38 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 56 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CCAACATGGAGCGCATCATG |
| Suggested primer 2: | TCTTCCTGGAGGCCTAGGTC |