| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.004257 |
| Chromosome: | chromosome 6 |
| Location: | 3248346 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277100 | (1 of 4) PF03435 - Saccharopine dehydrogenase NADP binding domain (Sacchrp_dh_NADP) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCTAAATTTCATTGTAATTGGGCCAGATAGGGCCTGCGGGGCTTGCAGCC |
| Internal bar code: | GTATCGTTGGGTTCACCATATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1453 |
| LEAP-Seq percent confirming: | 91.1765 |
| LEAP-Seq n confirming: | 31 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 34 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTGAGCACATCCACATCCCC |
| Suggested primer 2: | GGAACTGAGGAGTGTGGTGG |