Insertion junction: CLIP2.004267_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.004267
Chromosome:chromosome 15
Location:5485422
Confidence (%):63
Locus systematic id Locus common name Defline Feature
Cre17.g733650 BIO3,BIOF 7-keto-8-aminopelargonic acid synthase; (1 of 1) K00652 - 8-amino-7-oxononanoate synthase (bioF) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACACACGCCACCCTGACACGCACCTGGTCCGCCACGCCCGCCGCCTCCGC
Internal bar code:CCTGTGGTTCCGCCGGGGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:340
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:1
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:1

Suggested primers for confirmation by PCR

Suggested primer 1:GTACACGCCGGGGTATGTAG
Suggested primer 2:GCGAGGTGCATCGAAATGAC