| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004274 |
| Chromosome: | chromosome 3 |
| Location: | 5542900 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g185150 | (1 of 11) IPR023109 - Integrase/recombinase, N-terminal | 3'UTR | |
| Cre03.g185200 | CPL3,MPA6 | (1 of 3) PTHR11668//PTHR11668:SF267 - SERINE/THREONINE PROTEIN PHOSPHATASE // SUBFAMILY NOT NAMED; protein ser/thr phosphatase | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGCTCCAATGCTCAAGTACACAGCTTGATGCCTCGGTTTTCTACTAGCAT |
| Internal bar code: | ATACATATGTTCCTACCGTGGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2001 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 90 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGTTTCCTAGGACTGT |
| Suggested primer 2: | TGCATGTTGTTTGGTGCTCG |