| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004280 |
| Chromosome: | chromosome 7 |
| Location: | 5281936 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g349700 | Tic62-NAD-2 | (1 of 2) PTHR14194//PTHR14194:SF71 - NITROGEN METABOLIC REGULATION PROTEIN NMR-RELATED // PRESTALK A DIFFERENTIATION PROTEIN A; Putative dehydrogenase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGTCACACGGTCTTTGCCCGCGGGAGGGTTTGCAGCAAAGTCGGGGTTG |
| Internal bar code: | AACTTGATTTCTCCGTCGTCTC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2043 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 97 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 97 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTCTGCCGTCAACTGGTTC |
| Suggested primer 2: | TCTACATGGCAGCCGTTTGT |