| Insertion cassette: | CIB2 |
| Side of cassette: | 3' truncated? |
| Strand: | + |
| Strain: | CLIP2.004314 |
| Chromosome: | chromosome 9 |
| Location: | 2706993 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g391650 | HCP4 | (1 of 4) 1.7.99.1 - Hydroxylamine reductase / Hydroxylamine (acceptor) reductase; Hybrid-cluster protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATGTAAGCCGTACAAATTGGTTGCATTGCTCATCTGTTCAACACGCTCTC |
| Internal bar code: | CGGGGCAATTTTGTCCGAGTCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1772 |
| LEAP-Seq percent confirming: | 69.697 |
| LEAP-Seq n confirming: | 46 |
| LEAP-Seq n nonconfirming: | 20 |
| LEAP-Seq n unique pos: | 66 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTATCCAGGAGAAGCGGCTG |
| Suggested primer 2: | TCCTCAAAAGCGGAGACACC |