| Insertion cassette: | CIB2 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | CLIP2.004318 |
| Chromosome: | chromosome 6 |
| Location: | 5884140 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g289600 | (1 of 781) IPR000104 - Antifreeze protein, type I | 3'UTR | |
| Cre06.g289650 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACAGCTCACAGTTCACGGAACACGGTAATATTCACTTGTTCATGATGCA |
| Internal bar code: | ATCATTTAGCATCGTACGACAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 4743 |
| LEAP-Seq percent confirming: | 82.2222 |
| LEAP-Seq n confirming: | 37 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 45 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGAACATTTGGGTGCATGGG |
| Suggested primer 2: | CGAGGATGTGACTGTGGGTC |