| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004319 |
| Chromosome: | chromosome 10 |
| Location: | 4660428 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g451900 | THS1 | Threonine synthase; (1 of 1) 4.2.3.1 - Threonine synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TATTCGCTCCACACGCCCCGGCACCAAAGGTTCGGGGCCCGAACAAAGCA |
| Internal bar code: | TGACAATGCCACGTTCGCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6284 |
| LEAP-Seq percent confirming: | 98.1481 |
| LEAP-Seq n confirming: | 106 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 108 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACCAGCTCTTTTGCCTGGTT |
| Suggested primer 2: | TGCTATGTTGCTGTGCCAGA |