| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004323 |
| Chromosome: | chromosome 12 |
| Location: | 8275167 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g551350 | LAO2 | (1 of 1) 3.5.99.5 - 2-aminomuconate deaminase; Periplasmic L-amino acid oxidase, accessory subunit | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGAGGGAGGTGGGCAGGCGGGTCAGGAGGAAAGCAACTTGGGTATACAG |
| Internal bar code: | CGTTGATTATTGATGTTGCGAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 815 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 16 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 16 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTGCATACGACATGCTGGC |
| Suggested primer 2: | CGCACATACACGGCATCTTG |