Insertion junction: CLIP2.004361_1

Overview

Insertion cassette:CIB2
Side of cassette:3'
Strand:-
Strain:CLIP2.004361
Chromosome:chromosome 16
Location:7852158
Confidence (%):80
Locus systematic id Locus common name Defline Feature
Cre16.g689087 RTN1 (1 of 1) PTHR10994//PTHR10994:SF71 - RETICULON // RETICULON-LIKE PROTEIN B3; related to reticulon CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TCCAAAACAGCGAGGCCAGCAGCACGTAGCACAGGCCTGGACAGGGCAGG
Internal bar code:AGTATGAATAACCGTTTGATCG

Confirmation - LEAP-Seq

LEAP-Seq distance:2672
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:24
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:24

Suggested primers for confirmation by PCR

Suggested primer 1:ACGGCTGATGATAGCTTCGG
Suggested primer 2:ACGAGTAGTGGCGTTGAAGG