| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004365 |
| Chromosome: | chromosome 1 |
| Location: | 2537132 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g015000 | NAGK1,AGK1 | (1 of 1) 2.7.2.8 - Acetylglutamate kinase; N-acetyl-l-glutamate kinase | 3'UTR |
| Cre01.g015050 | AGK1 | (1 of 1) IPR006569//IPR017956//IPR020478 - CID domain // AT hook, DNA-binding motif // AT hook-like | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGCACCCGGGAAGCCTACGTTTCCGGATAACTTATTGTGATGGTTGGTTT |
| Internal bar code: | TATTGCGTCGTTTCATCGGTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 2578 |
| LEAP-Seq percent confirming: | 100.0 |
| LEAP-Seq n confirming: | 29 |
| LEAP-Seq n nonconfirming: | 0 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TCTTAACCCGACACCCGTTG |
| Suggested primer 2: | CACGTCTGCAAGTGTGTGTG |