| Insertion cassette: | CIB2 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | CLIP2.004371 |
| Chromosome: | chromosome 2 |
| Location: | 508850 |
| Confidence (%): | 80 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g076600 | Putative chloroplast localized Peptidyl-tRNA hydrolase; (1 of 1) K01056 - peptidyl-tRNA hydrolase, PTH1 family [EC:3.1.1.29] (PTH1, pth, spoVC) | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAATAAAAAAGATTTTGGGTTGGTTTAAGGACTCTGGACGCAGCCTGCAG |
| Internal bar code: | GTTCGGACAATTAAATAGCCGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 3009 |
| LEAP-Seq percent confirming: | 95.1219 |
| LEAP-Seq n confirming: | 39 |
| LEAP-Seq n nonconfirming: | 2 |
| LEAP-Seq n unique pos: | 41 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGCTTGCGGCAGTCAATAC |
| Suggested primer 2: | CTTCGCCTGAGCTACTACCG |